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Spectrophotometry is one of the most important practices carried out in physics laboratories. Spectrophotometry is the study of the reflection or transmission properties of a substance as a function of wavelength. It is the quantitative study of electromagnetic spectra of a material. During the process, the transmittance or reflectance of the substance is measured through the careful geometrical and spectral consideration.

To carry out spectrophotometry, a spectrophotometer is required. A spectrophotometer is a device or more specifically a photometer that is used to measure intensity of light as a function of the wavelength of light. Different types of spectrophotometers are found in the market. They are classified on various basis. The most important distinctions used to classify them are:

Principle of Spectrophotometry

A spectrophotometer, one of the most useful physics lab equipments is the combination of two devices, a spectrometer and a photometer. Spectrometer is used for producing light of any selected wavelength or color while a photometer is used for measuring the intensity of light. The two devices are placed at either side of a cuvette filled with a liquid. Spectrometer produces the light of desired wavelength and it passes through the tube and reaches photometer that measures its intensity. Then the photometer produces a voltage signal to a display device, usually a galvanometer. As the amount of light absorbed by the liquid changes the signal also changes. The concentration of a substance in solution can be measured by calculating the amount of absorption of light at the appropriate wavelength or a particular color.

I am a laboratory analyst by profession working in this industry from last 10 years. I have been running a site http://www.laboratoryequipmentworld.com/ on laboratory equipments. It includes the largest range of laboratory apparatus and other updated info on market, trade fairs, news, etc.

distinguishing between linkage isomers using IR spectroscopy.?

Linkage isomers
nitropentaamminecobalt(iii)chloride shows 2 bands at 1065 and 1470
nitritopentaamminecobalt(iii)chloride shows 2 bands at 1300 and 1430
on infrared spectrum.

Look at how the NO2 group is coordinated to the metal

Nitro is coordinated by the N with two oxygen which can have symmetric/asymmetric stretches while the nitrito is coordinated through an oxygen O-N-O.

If you draw it out I'm sure the answer will become clear!

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